Faculty

The focus of this lab is to understand the how the cytoskeleton affects cell signaling and cell structure/function.  Specifically, we are interested in the function of the EMAP-like protein (ELP) family of microtubule-binding proteins and how these proteins affect the function of muscle cells and sensory neurons in the nematode Caenorhabditis elegans. We take an integrated and multidisciplinary approach that includes proteomics, genomics, cell biology, molecular genetics and advanced imaging techniques.

Representative Publications

 

Eichenmuller B, Everley P, Palange J, Lepley D, Suprenant KA. 2002.

The human EMAP-like protein-70 (ELP70) is a microtubule destabilizer that localizes to the mitotic apparatus. J Biol Chem. 277(2):1301-1309.

Bode CJ, Gupta ML Jr, Reiff EA, Suprenant KA, Georg GI, Himes RH. 2002.

Epothilone and paclitaxel: unexpected differences in promoting the assembly and stabilization of yeast microtubules. Biochemistry 41:3870-3874.

Gupta ML Jr, Bode CJ, Thrower DA, Pearson CG, Suprenant KA, Bloom KS, Himes RH. 2002

beta-Tubulin C354 Mutations that Severely Decrease Microtubule Dynamics Do Not Prevent Nuclear Migration in Yeast. Mol Biol Cell. 13:2919-2932.

Suprenant KA. 2002

Vault ribonucleoprotein particles: sarcophagi, gondolas, or safety deposit boxes? Biochemistry41(49):14447-54.

Bode CJ, Gupta ML, Suprenant KA, Himes RH. 2003

The two alpha-tubulin isotypes in budding yeast have opposing effects on microtubule dynamics in vitro. EMBO Rep. 4(1):94-9.

Eichenmüller, B., Kedersha, N.,  Solovyeva, E.  Everley, P.,  Lang, J., Himes, R.H., and Suprenant, K.A. (2003)

Vaults bind directly to microtubules via their caps and not their barrels. Cell Motility and the Cytoskeleton 56:225-236.

Foland TB, Dentler WL, Suprenant KA, Gupta ML Jr, and Himes RH. 2005

Paclitaxel-induced microtubule stabilization causes mitotic block and apoptotic-like cell death in a paclitaxel-sensitive strain of Saccharomyces cerevisiae. 22:971-8. PMID: 16134117 [ in process]

Rodriguez AJ, Seipel SA, Hamill DR, Romancino DP, DI Carlo M, Suprenant KA, and Bonder EM. 2005.

Seawi--a sea urchin piwi/argonaute family member is a component of MT-RNP complexes. RNA. 11:646-56. PMID: 15840816

Stewart PL, Makabi M, Lang J, Dickey-Sims C, Robertson AJ, Coffman JA, Suprenant KA. 2005.

Sea urchin vault structure, composition, and differential localization during development. BMC Dev Biol. 2005 5(1):3. PMID: 15710043 [ in process]

Suprenant, K.A., Bloom, N., Fang, J. and Lushington, G. (2007)

The major vault protein is related to the toxic anion resistance protein (TelA) family. Journal of Experimental Biology.210:946-955.**

PMID: 17337707 [PubMed - in process]

** Featured in Inside JEB section with an article entitled: The Mystery of Vaults.